Issue 11/2025
Denchev, V., Handjieva-Darlenska, T.
Department of Pharmacology and Toxicology, Medical Faculty, Medical University – Sofia
At the present time, obesity is one of the most significant diseases, which negatively affects heath on a societal level, worsens the quality of life of patients, and is associated with a risk of a large number of complications affecting many different organs and systems. A lot of different factors contribute to the pathogenesis of obesity, and the main one is hypercaloric diet with high content of rapidly absorbed monosaccharides and saturated fatty acids. The aim of our current research was to induce obesity in laboratory animals, by conducting the two following research models. In the first experiment adult male Wistar rats (n=45) were randomly divided into three groups: Group 1 (n=15), control, Group 2 (n=15), receiving a standard diet with additional 25% solution of glucose, Group 3 (n=15), receiving a standard diet with additional 25% solution of fructose, for the duration of 20 weeks. In the second experiment adult male Wistar rats (n=20) received diet with high content of fat with additional 25% solution of fructose, for the duration of 12 weeks. In order to establish the effectiveness of application of both diets during the two experiments, biometric indicator data was obtained from the laboratory animals – body weight, abdominal circumference, body-mass index and Lee index.
Key words: obesity, metabolism, experimental model, research animals
Address of correspondence:
Dr. V. Denchev,
Prof. Dr. T. Handzhieva-Darlenska, MD
Faculty of Medicine, Medical University – Sofia
Department of Pharmacology and Toxicology,
Medico-Biological Complex, 3rd floor,
2 Zdrave Str.,
1431, Sofia
e-mail: v.denchev@medfac.mu-sofia.bg
e-mail: tdarlenska@medfac.mu-sofia.bg